A novel Golgi mannosidase inhibitor: Molecular design, synthesis, enzyme inhibition, and inhibition of spheroid formation, Ryosuke Koyama, Yui Kano, Kaori Kikushima, Ayaka Mizutani, Yuta Soeda, Kazuki Miura, Takako Hirano, Toshiyuki Nishio, Wataru Hakamata, Bioorganic & Medicinal Chemistry, 10, 115492 (2020). [Direct link] 

Development of Fluorogenic Substrates of α-L-Fucosidase Useful for Inhibitor Screening and Gene-expression Profiling, K. Miura, T. Tsukagoshi, T. Hirano, T. Nishio, W. Hakamata, ACS Medicinal Chemistry Letters, 10, 1309-1313 (2019). [Direct link]

精密分子設計に基づく蛍光基質の開発とそれを用いた新規糖質加水分解酵素の探索と同定、三浦一輝、袴田　航、西尾俊幸、9(3)，189-194, (2019).

用語解説　アノマー効果、袴田　航、応用糖質科学、9(1)，57-58, (2019).

Chitin heterodisaccharide, released from chitin by chitinase and chitin oligosaccharide deacetylase, enhances the chitin-metabolizing ability of Vibrio parahaemolyticus, T. Hirano, M. Okubo, H. Tsuda, M. Yokoyama, W. Hakamata, T. Nishio, Journal of Bacteriology, 201(20), e00270-19 (2019). [Direct link]

Antiviral Activity and Mechanism of Action of Endoplasmic Reticulum Glucosidase Inhibitors: A Mini Review, M. Onda and W. Hakamata, Trends in Glycoscience and Glycotechnology,  30(176), E139-E145 (2018). Free for all readers!  DOI

＊Trends in Glycoscience and Glycotechnology はバイリンガル（英語と日本語）雑誌となっていますので、日本語で読みたい方は以下をクリック＊

↓↓↓↓↓↓

小胞体グルコシダーゼ阻害による抗ウイルス活性発現機序、恩田桃子、袴田　航、Trends in Glycoscience and Glycotechnology, 30(176), J115-J121 (2018). Free for all readers!  DOI

Identification of Small-Molecule Inhibitors of Human Golgi Mannosidase via a Drug Repositioning Screen. Koyama R, Hakamata W, Hirano T, Nishio T. Chem. Pharm. Bull. (Tokyo). 66 (6), 678-681, (2018). Free for all readers!  DOI

Synthesis of All Stereoisomers of 1-(4-Methoxyphenyl)-2,3,4,9-tetrahydro-N-methyl-1Hpyrido[3,4-b]indole-3-carboxamide. M. Onda, A.Higashida, T. Hirano,  T. Nishio, W. Hakamata, Molbank. 2018(1), M973, (2018). DOI

Design and synthesis of cell-permeable fluorescent nitrilotriacetic acid derivatives. Tsuji G, Hattori T, Kato M, Hakamata W, Inoue H, Naito M, Kurihara M, Demizu Y, Shoda T. Bioorg. Med. Chem., 26 (20), 5494-5498, (2018). DOI

Synthesis of Chitin Oligosaccharides Using Dried Stenotrophomonas maltophilia Cells Containing a Transglycosylation Reaction-Catalyzing β-N-Acetylhexosaminidase as a Whole-Cell Catalyst. Uehara A, Takahashi N, Moriyama M, Hirano T, Hakamata W, Nishio T. Appl. Biochem. Biotechnol., 184 (2), 673-684, (2018). DOI 

Chemoenzymatic synthesis of sucuronic acid using d-glucurono-6,3-lactone and sucrose as raw materials, and properties of the product. Hosaka H, Mizoguchi S, Tashiro M, Fujimoto T, Hirano T, Hakamata W, Nishio T. Enzyme Microb. Technol. , 110, 53-60, (2018). DOI 

スクロースアナログ二糖のヒト腸内ビフィズス菌および乳酸菌における増殖効果、保坂浩貴、永嶋誠、平野貴子、袴田航、西尾俊幸、精糖技術研究会誌, 64, 5-10 (2018).

In vivo programming of endogenous antibodies via oral administration of adaptor ligands. Nagano M., Carrillo ., Otsubo N., Hakamata W., Ban H., Fuller R.P., Bashiruddin N.K., Barbas CF 3rd. Bioorg. Med. Chem., 25 (21), 5952-5961, (2017). DOI

糖鎖合成阻害を機序とする薬剤耐性回避型抗ウイルス剤の開発、小山亮祐、袴田航、月刊「細胞」、49 (14), 720-724 (2017). Link

 ＊この論文は好評でしたので、編集部の意向により下記の雑誌にも同内容で掲載されています＊

↓↓↓↓↓↓

糖鎖合成阻害を機序とする薬剤耐性回避型抗ウイルス剤の開発、小山亮祐、袴田航、アグリバイオ、1 (14), 1488-1492 (2017). Link

スクロース構造に着目した特殊二糖の酵素合成、保坂浩貴、溝口早織、平野貴子、袴田航、西尾俊幸、精糖技術研究会誌, 63, 11-15 (2017).

1,3-Butanediol Dibenzoate, Hakamata W, Goto K, Ishiwata S, Ouki R, Sakai R, Iizuka A, Yano S, Hirano T, Nishio T., Molbank, 2016 (3), M905, (2016). DOI  Open Access

Discovery of human Golgi β-galactosidase with no identified glycosidase using a QMC substrate design platform for exo-glycosidase. Miura K, Hakamata W, Tanaka A, Hirano T, Nishio T, Bioorg. Med. Chem., 24 (6), 1369-1375, (2016).  DOI

Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine and melibiose using Aspergillus niger alpha galactosidase, and properties of the products. Sakaki Y, Tashiro M, Katou M, Sakuma C, Hirano T, Hakamata W, Nishio T. Biosci. Biotechnol. Biochem. 80 (9), 1836-1842, (2016). DOI

Enzymatic synthesis and the structure elucidation of novel trisaccharides comprised of N-acetyl-D-glucosamine, and D-fructos. Tashiro M., Fujimoto T., Furihata K., Sakai Y., Hirano T., Hakamata W., Nishio T. J. Carbohydr. Chem. 35 (7), 376-386, (2016). DOI

Chemoenzymatic synthesis and properties of sucuronamide. S. Mizoguchi, H. Hosaka, M. Tashiro, T. Hirano, W. Hakamata, T. NishioJ. Carbohydr. Chem35(8-9), 397-411, (2016).#69aa4b;">DOI

Chitin oligosaccharide deacetylase from Shewanella baltica ATCC BAA-1091 T. Hirano, H Shiraishi, M. Ikejima, R. Uehara, W. Hakamata, T. Nishio. Biosci. Biotechnol. Biochem. 80 (9), 1836-1842, (2016).DOI

酵素の構造に基づいた蛍光基質の開発とそれを用いた新しい糖鎖修飾酵素の発見、三浦一輝、袴田航、田中綾子、平野貴子、西尾俊幸、精糖技術研究会誌, 61, 27-32 (2016).

A Simple Synthesis of Alliin and allo Alliin: X-Ray Diffraction Analysis and Determination of their Absolute Configuration, Hakamata W, Koyama R, Tania M, Haga T, Hirano T, Akao M, Kumagai H, Nishio T, J. Agric. Food Chem, 63 (50), 10778-10784, (2015).  DOI

Identification of a novel glycan processing enzyme with exo-acting β-allosidase activity in the Golgi apparatus using a new platform for the synthesis of fluorescent substrates, Hakamata W, Miura K, Hirano T, Nishio T.,  Bioorg. Med. Chem. 23 (1), 73-79, (2015). DOI　コスモバイオ学術論文賞

Saccharification of beta-chitin from squid pen by fermentation method using recombinant chitinase-secreting Escherichia coli, Minamoto T, Takahashi T, Kitahara S, Shinozaki Y, Hirano T, Hakamata W, Nishio T, Appl. Biochem. Biotechnol. 175, 3788-3799, (2015). DOI

Chitin Oligosaccharide Deacetylase from Shewanella woodyi ATCC51908, Hirano T, Uehara R, Shiraishi H, Hakamata W, Nishio T, J. Appl. Glycosci. 62, 153-157, (2015). DOI 

Structure-based analysis of domain function of chitin oligosaccharide deacetylase from Vibrio parahaemolyticus, Hirano T, Sugiyama K, Sakai Y, Hakamata W, Park SY, Nishio T, FEBS Lett. 589 (1) 145-151, (2015). DOI

Vibrio 属細菌由来キチンオリゴ糖脱アセチル化酵素の諸性質・構造および生成糖の生理機能、平野貴子、杉山佳奈子、坂木祐太、上原理絵、白石晴香、袴田航、朴三用、西尾俊幸、応用糖質科学 5 (2), 113-119, (2015).

Multicolor Imaging of Endoplasmic Reticulum-Located Esterase As a Prodrug Activation Enzyme, Hakamata W,  Tamura S, Hirano T, Nishio T, ACS Medicinal Chemistry Letters, 5 (4), 321–325 (2014). DOI

Detection of N-linked oligosaccharide processing enzyme activity at cellular level, Hakamata W, Bull. Appl. Glycosci., 3, 195-196 (2013).

Facile and stabile linkages through tyrosine: bioconjugation strategies with the tyrosine-click reaction, H. Ban, M. Nagano, J. Gavrilyuk, W. Hakamata, T. Inokuma, C.F. Barbas, Bioconjugate Chem., 24, 520-532 (2013). DOI

Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine using β-fructofuranosidase from Aspergillus oryzae. T. Nishio, M. Juami, T. Wada, Y. Sugimoto, H. Senou, C. Sakuma, T. Hirano, W. Hakamata, M. Tashiro, Carbohydr. Res., 382, 108-112 (2013). DOI

Chitin oligosaccharide deacetylase from Vibrio harveyi ATCC BAA-1116: gene cloning, overexpression, purification, and characterization, HiranoT, Maebara Y, Uehara R, Sakai Y, Shiraishi H, Ichimura S, Hakamata W, Nishio T, Chitin and Chitosan Research, 19 (3), 321-324 (2013).

スクロース類似構造を有する N-アセチルグルコサミン含有オリゴ糖である N-アセチルスクロサミンの生産　佐藤仁、和田徹、 岩井すみれ、 重網麻依、中村成寿、横地佐江子、 笠間輝美、 平野貴子、袴田航、西尾俊幸、精糖技術研究会誌、59, 41-51, ( 2013).

Virtual ligand screening ofα-glucosidase: Identification of a novel potent noncarbohydrate mimetic inhibitor. Hakamata W, Ishikawa R, Ushijima Y, Tsukagoshi T, Tamura S, Hirano T, Nishio T, Bioorg. Med.Chem. Lett., 22 (1) 62-64 (2012). DOI

Virtual Ligand Screening of α‐Glucosidase: Identification of a Novel Potent Noncarbohydrate Mimetic Inhibitor. Hakamata, W., Ishikawa, R., Ushijima, Y., Tsukagoshi, T., Tamura, S., Hirano, T., Nishio, T. ChemInform Selected abstract in Chemistry, 43 (20) (2012). DOI

Formylbenzene diazonium hexafluorophosphate reagent for tyrosine-selective modification of proteins and the introduction of a bioorthogonal aldehyde, J. Gavrilyuk, H. Ban, M. Nagano, W. Hakamata, C. F. Barbas, Bioconjugate Chem., 23, 2321-2328 (2012). DOI

Continuous Production of β-D-Fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-D-glucopyranoside (N-Acetylsucrosamine) Using a Column Reactor Packed with β-Fructofuranosidase-containing Mycelia of Aspergillus oryzae Immobilized on a Porous Carrier, Sato Η, Yokochi S, Kasama T, Hirano T, Hakamata W, Nishio T, J. Appl. Glycosci,, 59 (4), 153-160, (2012). DOI

Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium hermophilum: Characterization using recombinant protein, Yasuko Kumaki, Masahiro Ogawa, Takako Hirano, Keiko Yoshikawa, Naoya Iwasawa, Takurou Yagi, Wataru Hakamata, Tadatake Oku, Toshiyuki Nishio, Journal of Bioscience and Bioengineering, 111 (2), 134-139 (2011). DOI

Study of substrate specificity for glycosidase inhibitor design. Wataru Hakamata, Bulletin of Applied Glycoscience. 1 (1), 51-57 (2011). DOI

Design and synthesis of a ER-specific fluorescent probe based on carboxylesterase activity with quinone methide cleavage process, Wataru Hakamata, Aki Machida, Tadatake Oku, Toshiyuki Nishio, Bioorganic and Medicinal Chemistry Letters, 21 (11) 3206-3209 (2011). DOI

Involvement of CarA/LitR and CRP/FNR Family Transcriptional Regulators in Light-Induced Carotenoid Production in Thermus thermophilus, Hideaki Takano, Masato Kondo, Noriyoshi Usui, Toshimitsu Usui, Hiromichi Ohzeki, Ryuta Yamazaki, Misato Washioka, Akira Nakamura, Takayuki Hoshino, Wataru Hakamata, Teruhiko Beppu, and Kenji Ueda, The Journal of Bacteriology, 193 (10), 2451-2459, (2011). DOI

Heterodisaccharide 4-O-(N-acetyl-β-D-glucosaminyl)-D-glucosamine is an effective chemotactic attractant for Vibrio bacteria that produce chitin oligosaccharide deacetylase, T. Hirano, M. Aoki, K. Kadokura, Y. Kumaki, W. Hakamata, T. Oku, T. Nishio, Letters in Applied Microbiology, 53 (2), 161–166,(2011). DOI

α-グルコシダーゼ阻害剤開発における酵素情報の重要性、袴田　航、化学工業、61 (120), 927-931, (2010).

Expression, purification, physicochemical characterization and structural analysis of cytochrome c554 from Vibrio parahaemolyticus strain RIMD2210633. Akazaki, Hideharu; Kawai, Fumihiro; Chida, Hirotaka; Hirano, Takako; Hakamata, Wataru; Park, Sam-Yong; Nishio, Toshiyuki; Oku, Tadatake, Bioscience, Biotechnology, and Biochemistry, 74 (5), 1113-1115, (2010).

Suicide Substrate-based Inactivation of Endodextranase by ω-Epoxyalkyl α-D-Glucopyranosides. Hee-Kwon Kang, Young-Min Kim, Hiroyuki Nakai, Min-Sun Kang, Wataru Hakamata, Masayuki Okuyama, Haruhide Mori, Toshiyuki Nishio, Atsuo Kimura, Journal of Applied Glycoscience, 57 (4), 269-272, (2010).

Design and Screening Strategies for α-Glucosidase Inhibitor Based on Enzymological Information. Wataru Hakamata, Masaaki Kurihara, Haruhiro Okuda, Toshiyuki Nishio, Tadatake Oku, Current Topics in Medicinal Chemistry, 9, 3-12, (2009).

Crystallization and structural analysis of cytochrome c6 from the diatom Phaeodactylum tricornutum at 1.5 A resolution. Akazaki, H., Kawai, F, Hosokawa, M., Hama, T., Chida, H., Hirano, T., Lim, B. K., Sakurai, N., Hakamata, W., Nishio, T., Park, S. Y. and Oku, T. Bioscience, Biotechnology, and Biochemistry, 73, 189-191, (2009).

Heterodisaccharide 4-O-(N-acetyl-β-D-glucosaminyl)-D-glucosamine is a specific inducer of chitinolytic enzyme production in Vibrios harboring chitin oligosaccharide deacetylase genes. Takako Hirano, Kazunari Kadokura, Takanori Ikegami, Yuko Shigeta, Yasuko Kumaki, Wataru Hakamata, Tadatake Oku, Toshiyuki Nishio, Glycobiology, 19 (9), 1046-1053, (2009).

A chitin oligosaccharide deacetylase from Vibrio sp. SN184: Gene Cloning, overexpression, purification, and characterization. Sakamoto, Yusuke; Kuno, Eriko; Tomiyama, Ayumi; Hirano, Takako; Kumaki, Yasuko; Tanaka, Akina; Kanda, Hiroshi; Furuya, Kenichi; Hakamata, Wataru; Oku, Tadatake, Journal of Applied Glycoscience, 56 (4), 273-276, (2009).